Description: This is an image of a copepod collected from the ocean. Orange is membrane stain and brings out the autofluorescence of the chitin exoskeleton. Nuclei have been stained with Hoescht and is shown in a rainbow of depth code to reflect different heights in the image
Zachary Sanchez
Instrumentation: Yokogawa W1 Spinning disk confocal, Nikon Ti2 Microscope, 60x oil objective, Luemcor ZIVA light source and Hamamatsu BT Fusion camera
Description: A kymograph of a five-hour video recording the nervous system development in a zebrafish embryo whose neurons are labeled by ngn1:gfp-caax, a neuron-specific transgenic reporter. Time points are color-coded, using the Temporal-Color Code function in FIJI.
Instrumentation: imaging was performed on the Nikon/Yokogawa SoRa with Nikon Plan Apo 40x/0.95 lens, Hamamatsu Fusion BT camera.
Instrumentation: 10x objective, Nikon CSU-W1, Hamamatsu Fusion BT camera
Kate McCluskey
Brain Veins
Transgenic C. elegans expressing endogenously tagged laminin (neon GFP) and collagen IV (mKate) which compose the basement membranes that outlines various organ structures in the nematode.
Instrumentation: Image acquired with Plan Apo 60x NA 1.40 lens Nikon on the microscope Nikon Eclipse with W1 Yokogawa SD-unit and Nikon Elements software. Hamamatsu Fusion BT camera
Lucrezia C. Ferme
Drawing animal outlines using the basement membranes
Description: Axolotl pre-hatchling with DAPI (Blue) , anti-Serotonin (Yellow) and Actin labelled (Magenta)
Instrumentation: 10x with Nikon Ti2 with CSU-W1 using Nikon Elements software, Hamamatsu Fusion BT camera
Instrumentation: 10x with Nikon Ti2 with CSU-W1 using Nikon Elements software, Hamamatsu Fusion BT camera
Verena Kaul
Tiny Tentacles, Big World
Stuck Together: Cyanobacteria and Bacteria’s ride-or-die alliance
Chih-Hsuan Hsu, Xiangyi Ding, Carolina Trenado-Yuste, Alejandro Martínez-Calvo
Description: Nostoc (orange), a filamentous multicellular cyanobacterium, forms symbiotic relationships with motile bacteria (green). This micrograph captures the metabolic interactions between bacteria and cyanobacteria in a nutrient-poor, highly confined 3D environment. Neither can survive in nutrient-poor conditions on their own but when co-cultured with cyanobacteria, they proliferate and thrive together, indicating a beneficial metabolic relationship.
Instrumentation: Nikon W1-SoRa spinning disk confocal using PLAN APO λD 60x oil objective lens, Hamamatsu Fusion BT camera
Powering the Next Generation
Tamas Nagy
Description: Mitochrondrial marker TOMM20-GFP (Blue) in C. elegans
Instrumentation: 60x oil objective on the Nikon Ti2 + W1 + Nikon Elements software.
Symbiotic Dance: Cyanobacteria and Their Motile Partners
Chih-Hsuan Hsu, Xiangyi Ding, Carolina Trenado-Yuste, Alejandro Martínez-Calvo
Description: Motile bacteria, Escherichia coli (green), interact with the non-motile filamentous cyanobacterium, Nostoc muscorum (orange). E. Coli use their mobility to swim towards cyanobacteria to uptake nutrients for growth, thus moving the Nostoc around forming a symbiotic microbial relationship .
Instrumentation: Nikon W1-SoRa spinning disk confocal, PLAN APO λD 60x OIL OFN25 DIC N2 objective lens.
lightning nerves (the movie!!!)
Ruth Styfhals, Jakke Neiro, Ruoyu Chen
Description: A video of nervous system development in a zebrafish embryo. Neurons are labeled by ngn1:gfp-caax, a neuron-specific transgenic reporter. The embryo was 28 hours to 32 hours post fertilization.
Instrumentation: Imaging was performed on the Nikon/Yokogawa SoRa with Nikon Plan Apo 40x/0.95 lens.
Description: E. coli bacteria (green) interact with and sort the rod-shaped unicellular cyanobacterium, Synechococcus elongatus (magenta). Each bacteria type self-organize, occupying distinct spatial domains. Researching such interactions can enhance our knowledge of microbial behavior and how they coexist and support each other in challenging environments.
Instrumentation: Photo was taken on Nikon/ Yokogawa W1 spinning disk confocal using Plan Apo λ 60x Oil objective lens
Microbial Mosaic: The Separation Spectacle of Cyanobacteria and Bacteria
Chih-Hsuan Hsu, Carolina Trenado-Yuste, Alejandro Martínez-Calvo
Description: Motile E. coli bacteria (green) interact with and sort the rod-shaped unicellular cyanobacterium, S. elongatus (orange). Each bacteria type self-organize, occupying distinct spatial domains capturing the community interactions between motile bacteria and cyanobacteria in nutrient-poor aquatic environments.
Instrumentation: The photo was taken on Nikon/ Yokogawa W1 spinning disk confocal using PLAN APO λD 20x OFN25 DIC N2 objective lens.
